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Antibodies

His-Tag Monoclonal Antibody

Product Sizes
25 ul
£97.00
E-AB-48003-25UL
100 ul
£171.00
E-AB-48003-100UL
About this Product
SKU:
E-AB-48003
Additional Names:
HHHHHH epitope tag; HHHHHH tag; Hexa His tag; His tag; Polyhistidine Tag;6 His epitope tag
Application:
Flow Cytometry, Immunoprecipitation, Western Blot
Buffer:
PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4
translate.label.attr.clone:
1A5
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world.
Host:
Mouse
Immunogen:
Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin.
Isotype:
IgG2a
Purification:
Affinity Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Tagged Antibodies
Western blot of His-Tag fused recombinant protein with Anti-His mouse monoclonal antibody at dilution of 1:1000.
IP Result of 293F cells transfected with His-Tag fusion protein,using anti-His-Tag mouse monoclonal antibody . Lane 1:input, Lane 2: anti-His-Tag mouse monoclonal antibody, Lane 3: mouse IgG1 Isotype Control
1x10^6 CHO cells Transfected with a His plasmid were stained with 0.2ug Anti-His tag mAb(E-AB-48003) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)