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Antibodies

HIF1 bata Monoclonal Antibody

Product Sizes
20 ul
£118.00
E-AB-22189-20UL
60 ul
£183.00
E-AB-22189-60UL
120 ul
£272.00
E-AB-22189-120UL
200 ul
£420.00
E-AB-22189-200UL
About this Product
SKU:
E-AB-22189
Additional Names:
ARNT;ARNT protein;Aryl hydrocarbon receptor nuclear translocator;Class E basic helix-loop-helix protein 2;Dioxin receptor;Dioxin receptor nuclear translocator;Drnt;HIF 1 beta;HIF 1beta;HIF-1-beta;HIF1-beta;HIF1B;HIF1beta;Hypoxia Inducible Fact;arnT;bHLHe2
Application:
IHC-Paraffin, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
translate.label.attr.clone:
4C5
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1A Alpha and HIF-1B Beta, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1A Alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1A Alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1A Alpha degradation and lead to its stabilization. In addition, HIF-1A Alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1B Beta is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1B Beta plays an important role in xenobiotics metabolism (8).
Host:
Mouse
Immunogen:
Recombinant Protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody