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Antibodies

Recombinant Cdc27 Monoclonal Antibody

Product Sizes
50 ul
£281.00
AN301730L-50UL
100 ul
£402.00
AN301730L-100UL
About this Product
SKU:
AN301730L
Additional Names:
NUC,APC,CDC,ANAPC,CDC27,ANAPC3,APC3,CDC27Hs,D0S1430E,D17S978E,H-NUC,HNUC,NUC2,Anaphase Promoting Complex 3,Anaphase promoting complex protein 3,Anaphase Promoting Complex Subunit 3,Anaphase-promoting complex subunit 3,APC 3,Cdc 27,CDC27 homolog,Cell Division Cycle 27,Cell division cycle protein 27 homolog,H NUC,Nuc2 homolog
Application:
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
translate.label.attr.clone:
A438
Clonality:
Recombinant Monoclonal
Concentration:
1 mg/ml
Extra Details:
Cell division cycle protein 27 homolog (CDC27) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Host:
Rabbit
Immunogen:
Recombinant human Cdc27 fragment
Isotype:
IgG, kappa
Purification:
Protein A Purified
Reactivities:
Human, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Recombinant Antibodies
Western Blot with Cdc27 Monoclonal Antibody at dilution of 1:5000. Lane 1: MCF-7, Lane 2: BRL
Immunohistochemistry of paraffin-embedded Human uterine cancer tissue using Cdc27 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using Cdc27 Monoclonal Antibody at dilution of 1:1000.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed 293T cells using anti-Cdc27 Monoclonal Antibody at dilution of 1:50.
Flow cytometric analysis of human Cdc27 expression on K562 cells. Cells were stained with purified anti-Human Cdc27, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.