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Antibodies

Recombinant CD34 Monoclonal Antibody

Product Sizes
20 ul
£189.00
AN300538P-20UL
100 ul
£402.00
AN300538P-100UL
About this Product
SKU:
AN300538P
Additional Names:
Cd34;gp105-120;Mucosialin
Application:
Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B468
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins (By similarity).
Host:
Rabbit
Immunogen:
Recombinant Mouse CD34 Protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry of paraffin-embedded mouse stomach using CD34 Monoclonal Antibody at dilution of 1:5000. Positive staining was localized to vascular endothelial cells.
Immunohistochemistry of paraffin-embedded mouse kidney using CD34 Monoclonal Antibody at dilution of 1:5000. Positive staining was localized to vascular endothelial cells.
Flow cytometric analysis of CD34 expression on M1 cells. Cells were stained with purified anti-Mouse CD34, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunoprecipitation analysis using 0.5 uL anti-Mouse CD34 Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using CD34 Monoclonal Antibody at a dilution of 1:350. Lane A:0.5 mg Hela Whole Cell Lysate, Lane B:0.5 mg 293T Whole Cell Lysate
Immunofluorescence analysis of CD34 in M1 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD34 monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).