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Antibodies

Recombinant CD5/Cluster of Differentiation 5 Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300514P-100UL
About this Product
SKU:
AN300514P
Additional Names:
Leu;Lyt;Lymphocyte antigen;Lymphocyte antigen T;T-cell surface glycoprotein CD;LEU1T-cell surface glycoprotein CD;CD5 antigen;CD5 antigen (p56-62);CD5 molecule;Leu-1;LEU1T-cell surface glycoprotein CD5;Lymphocyte antigen 1;Lymphocyte antigen T1;Lyt-1;T1;T-cell surface glycoprotein CD5;Ly-1;Cd5;Tp67;Ly-12;Ly-1
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B444
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature A Alpha/B Beta T cells express high levels of CD5, very few G Gamma/D Delta T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.
Host:
Rabbit
Immunogen:
Recombinant Mouse CD5/Cluster of Differentiation 5 protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Mouse CD5 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD5 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.
Flow cytometric analysis of Mouse CD5 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD5, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.