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Antibodies

Recombinant RhoA Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300421P-100UL
About this Product
SKU:
AN300421P
Additional Names:
ARH;RHOH;ARH12;ARHA;RHO12;RHOH12;Rho;RHOA;EDFAOB;ARH12;EDFAOB;RHO12;RHOH12;Aplysia ras related homolog 12;H 12;H12;Oncogene RHO H12;ras homolog family member A;Ras homolog gene family member A;Rho A;Rho cDNA clone 12;RHO H12;Small GTP binding protein Rho A;Transforming protein Rho A;Transforming protein RhoA
Application:
Immunocytochemistry, Immunofluorescence
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B347
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.
Host:
Rabbit
Immunogen:
Recombinant Human RhoA protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of RHOA in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human RHOA monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.