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Antibodies

Recombinant ARG1/Arginase 1 Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300403P-100UL
About this Product
SKU:
AN300403P
Additional Names:
ARGI;ARG;ARG1;arginase-1;A I;Al;ARG 1;ARGI1;Arginase;Arginase 1;Arginase I;Arginase liver;Arginase type I;Arginase1;liver;Liver Arginase;Liver type arginase;Liver-type arginase;Type I arginase;A I;Al;ARG 1;arg1;ARGI1;Arginase 1;Arginase liver;Arginase type I;Arginase;liver;Arginase-1;Arginase1;Liver type arginase;Liver-type arginase;Type I arginase;Arginase
Application:
Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B329
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion.
Host:
Rabbit
Immunogen:
Recombinant Human ARG1/Arginase 1 protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using ARG1/Arginase 1 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human kidney using ARG1/Arginase 1 Monoclonal Antibody at dilution of 1:1000.
Western Blot with ARG1 Monoclonal Antibody at dilution of 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.
Flow cytometric analysis of Human ARG1 expression on HepG2 cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human ARG1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of ARG1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ARG1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).