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Antibodies

Recombinant Peroxiredoxin 5/PRDX5 Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300395P-100UL
About this Product
SKU:
AN300395P
Additional Names:
PMP;ACR;AOEB;SBBI;Peroxiredoxin;Alu corepressor;Antioxidant enzyme B;Thioredoxin peroxidase PMP;HEL-S;PRDX5;ACR1;AOEB166;B166;HEL-S-55;PLP;PMP20;PRDX6;PRXV;SBBI10;prx-V;Alu corepressor 1;Antioxidant enzyme B166;Liver tissue 2D-page spot 71B;Peroxiredoxin V;Peroxiredoxin-5;Peroxisomal antioxidant enzyme;Thioredoxin peroxidase PMP20;Thioredoxin reductase;TPx type VI;Alu co repressor 1;epididymis secretory protein Li 55;mitochondrial;peroxiredoxin 5;PRDX 5
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B321
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene.
Host:
Rabbit
Immunogen:
Recombinant Human Peroxiredoxin 5/PRDX5 protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunoprecipitation analysis using 2 uL anti-PRDX5 Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using PRDX5 Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg Hela Whole Cell Lysate, Lane B:0.5 mg Jurkat Whole Cell Lysate, Lane C:0.5 mg 293T Whole Cell Lysate
Western Blot with PRDX5 Monoclonal Antibody at dilution of 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: 293 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.
Immunofluorescence analysis of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PRDX5 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Western Blot with PRDX5 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: PRDX5 konckout Hela Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lysates/proteins at 30 ug per lane.