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Antibodies

Recombinant Phospho-NF-K KappaB p65 (Ser536) Monoclonal Antibody

Product Sizes
20 ul
£167.00
AN300381L-20UL
100 ul
£402.00
AN300381L-100UL
About this Product
SKU:
AN300381L
Additional Names:
NFKB;Transcription factor p;Nuclear factor of kappa light polypeptide gene enhancer in B-cells;NF-kB p;NFKB3;p65;NF-kB p65;RELA;CMCU;Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;NFKB3;p65;transcription factor p65;Avian reticuloendotheliosis viral (v rel) oncogene homolog A;MGC131774;NF kappa B p65delta3;Nuclear Factor NF Kappa B p65 Subunit;Nuclear factor of kappa light polypeptide gene enhancer in B cells 3;OTTHUMP00000233473;OTTHUMP00000233474;OTTHUMP00000233475;OTTHUMP00000233476;OTTHUMP00000233900;p65 NF kappaB;p65 NFkB;TF65;V rel avian reticuloendotheliosis viral oncogene homolog A;v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65));V rel reticuloendotheliosis viral oncogene homolog A;v rel reticuloendotheliosis viral oncogene homolog A (avian)
Application:
Western Blot
Buffer:
10 mM sodium HEPES, 150 mM NaCl, 100 ug/mL protein protectant, 50% glycerol, pH 7.5
translate.label.attr.clone:
B307
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB.
Host:
Rabbit
Immunogen:
A synthetic phosphopeptide corresponding to residues around Ser536 of the Human NF-K KappaB p65
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (200 nM, 30 min) (line B); treated with Calyculin A and L Lambda-phosphatase (line C) using Phospho-NF-K KappaB p65 (Ser536) Antibody, Rabbit MAb at 1:1000 dilution.
Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-NF-K KappaB p65 (Ser536) Antibody, Rabbit MAb at 1:5000 dilution. (upper) or Beta-Tubulin Loading Control Antibody, Mouse MAb at 1:20000 dilution(lower).
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (200 nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-NF-K KappaB p65 (Ser536) Antibody, Rabbit MAb at 1:5000 dilution.
Western blot analysis of extracts from serum-starved NIH/3T3, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-NF-K KappaB p65 (Ser536) Antibody, Rabbit MAb at 1:1000 dilution.