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Antibodies

Recombinant Phospho-Chk1 (Ser345) Monoclonal Antibody

Product Sizes
20 ul
£167.00
AN300370L-20UL
100 ul
£402.00
AN300370L-100UL
About this Product
SKU:
AN300370L
Additional Names:
CHK;CHEK;CHEK1;CHK1
Application:
Western Blot
Buffer:
10 mM sodium HEPES, 150 mM NaCl, 100 ug/mL protein protectant, 50% glycerol, pH 7.5
translate.label.attr.clone:
B292
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Host:
Rabbit
Immunogen:
A synthetic peptide corresponding to residues around
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-Chk1 (Ser345) rabbit monoclonal Antibody at 1:5000 dilution. (upper) or Anti-beta-Actin Antibody Loading Control Antibody, Rabbit MAb (Chimeric) at 1:50000 dilution(lower).
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100 nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Chk1 (Ser345) rabbit monoclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with calyculin A (100 nM, 30min; +) (line B); treated with calyculin A and L Lambda-phosphatase (line C) using Phospho-Chk1 (Ser345) rabbit monoclonal Antibody at 1:1000 dilution.