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Antibodies

Recombinant Phospho-Histone H2A.X (Ser139) Monoclonal Antibody

Product Sizes
20 ul
£167.00
AN300358L-20UL
100 ul
£402.00
AN300358L-100UL
About this Product
SKU:
AN300358L
Additional Names:
H2A.X;H2A/X;H2AX;Histone H2AX;H2AFX;gamma H2A.X;G GammaH2AX
Application:
IHC-Paraffin, Western Blot
Buffer:
10 mM sodium HEPES, 150 mM NaCl, 100 ug/mL protein protectant, 50% glycerol, pH 7.5
translate.label.attr.clone:
B280
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Host:
Rabbit
Immunogen:
A synthetic peptide corresponding to residues around Ser139 of Human Phospho-Histone H2A.X
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer tissue using Histone H2A.X (Ser139) Monoclonal Antibody at dilution of 1:200.
Western blot analysis of extracts from serum-starved NIH-3T3, untreated(line A) or treated with UV (30min; +)(line B), using Phospho-Histone H2A.X (Ser139) rabbit monoclonal Antibody at 1:5000 dilution. (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution(lower).
Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30min; +) (line B); treated with UV and L Lambda-phosphatase (line C) using Phospho-Histone H2A.X (Ser139) rabbit monoclonal Antibody at 1:5000 dilution.
Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Histone H2A.X (Ser139) rabbit monoclonal Antibody at 1:5000 dilution.