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Antibodies

Recombinant P4HB/ERBA2L Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300349P-100UL
About this Product
SKU:
AN300349P
Additional Names:
PDIA;CLCRP;P4HB;CLCRP1;DSI;ERBA2L;GIT;P4Hbeta;PDI;PDIA1;PHDB;PO4DB;PO4HB;PROHB;Cellular thyroid hormone-binding protein;p55;Prolyl 4-hydroxylase subunit beta;Protein Disulfide-Isomerase
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B271
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
Host:
Rabbit
Immunogen:
Recombinant Human P4HB protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Human P4HB in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human P4HB monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to endoplasmic reticulum.
Immunoprecipitation analysis using 2 uL anti-P4HB Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using P4HB Monoclonal Antibody at a dilution of 1:200. Lane A:0.5 mg MCF-7 Whole Cell Lysate
Western Blot with P4HB Monoclonal Antibody at dilution of 1:100 dilution. Lane A: MCF7 Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.