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Antibodies

Recombinant JNK2/MAPK9 Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300348P-100UL
About this Product
SKU:
AN300348P
Additional Names:
MAPK;PRKM;JNK;MAPK9;JNK-55;JNK2;JNK2A;JNK2ALPHA;JNK2B;JNK2BETA;PRKM9;SAPK;SAPK1a;p54a;p54aSAPK;c Jun kinase 2;C Jun N terminal kinase 2;c-Jun N-terminal kinase 2;JNK 55;JNK2 alpha;JNK2 beta;Jun kinase;MAP kinase 9;MAPK 9;Mitogen activated protein kinase 9;mitogen-activated;Mitogen-activated protein kinase 9;MK09;Protein kinase;SAPK alpha;Stress activated protein kinase 1a;Stress-activated protein kinase JNK2
Application:
IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B270
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
Host:
Rabbit
Immunogen:
Recombinant Human JNK2/MAPK9 protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human MAPK9 monoclonal antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.
Immunohistochemistry of paraffin-embedded human kidney using JNK2/MAPK9 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human brain using JNK2/MAPK9 Monoclonal Antibody at dilution of 1:1000.
Western Blot with MAPK9 Monoclonal Antibody at dilution of 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lysates/proteins at 30 ug per lane.
Immunoprecipitation analysis using 0.5 uL anti-MAPK9 Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using MAPK9 Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg HepG2 Whole Cell Lysate, Lane B:0.5 mg Hela Whole Cell Lysate, Lane C:0.5 mg Jurkat Whole Cell Lysate, Lane D:0.5 mg MCF-7 Whole Cell Lysate