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Antibodies

Recombinant Angiopoietin-2/ANG 2 Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300346P-100UL
About this Product
SKU:
AN300346P
Additional Names:
angiopoitin,ANGP,Ang,ANGPT2,AGPT2,ANG2,AGPT 2,ANG 2,angiopoietin 2,Angiopoietin 2a,Angiopoietin 2B,Angiopoietin2,angiopoietin-2a,angiopoietin-2B,angiopoitin 2,ANGP2,ANGPT 2,Tie2 ligand,Tie2-ligand,Angiopoietin-2,ANG-2,Agpt2,ANG2,Angiopoietin 2a,Angiopoietin 2B,Angiopoietin2,ANGPT 2,Angpt2,Tie2 ligand
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
translate.label.attr.clone:
B268
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Involved in the regulation of lymphangiogenesis.
Host:
Rabbit
Immunogen:
Recombinant Human Angiopoietin-2/ANG 2 protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Human Ang2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Ang2 monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).
Western Blot with Ang2 Monoclonal Antibody at dilution of 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: A549 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.
Immunoprecipitation analysis using 0.5 uL anti-Ang2 Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using Ang2 Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg HepG2 Whole Cell Lysate, Lane B:0.5 mg A549 Whole Cell Lysate, Lane C:0.5 mg 293T Whole Cell Lysate