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Antibodies

Recombinant N-Cadherin/CD325/CDH2 Monoclonal Antibody

Product Sizes
20 ul
£189.00
AN300288P-20UL
100 ul
£402.00
AN300288P-100UL
About this Product
SKU:
AN300288P
Additional Names:
CDH;CDw;Cadherin 2 Type;cadherin;CD325;CDHN;CDw325;NCAD;N Cadherin;CDH2;Cadherin 2 Type 1;cadherin-2;N-Cadherin;CDH2 CDw325;cadherin-2;CD325;CDHN;CDw325;NCAD;CD325;CDHN;CDw325;NCAD;N Cadherin;CDH2
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B210
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Host:
Rabbit
Immunogen:
Recombinant Human N-Cadherin / CD325 / CDH2 Protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Flow cytometry analysis of N-Cadherin on HeLa cells. HeLa cells were harvested with (Left) or without (Right) trypsinization [please note, the epitope is sensitive to trypsin] and stained with either purified Rabbit IgG isotype control (dashed line) or Rabbit Anti-Human CD325 antibody at matching concentrations , then a FITC-conjugated second step antibody.
Immunofluorescence analysis of Human CDH2 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CDH2 Monoclonal Antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to membrane.
Western Blot with N-Cadherin / CD325 / CDH2 Monoclonal Antibody at dilution of 1:500. Lane A: Hela Whole Cell Lysate, Lysates/proteins at 30 ug per lane.
Western Blot with N-Cadherin / CD325 / CDH2 Monoclonal Antibody at dilution of 1:500. Lane A: 293T Whole Cell Lysate, Lane B: N Cadherin konckout 293T Whole Cell Lysate, Lysates/proteins at 30 ug per lane.