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Antibodies

Recombinant N-Cadherin/CD325/CDH2 Monoclonal Antibody

Product Sizes
20 ul
£189.00
AN300288P-20UL
100 ul
£402.00
AN300288P-100UL
About this Product
SKU:
AN300288P
Additional Names:
CDH,CDw,Cadherin 2 Type,cadherin,CD325,CDHN,CDw325,NCAD,N Cadherin,CDH2,Cadherin 2 Type 1,cadherin-2,N-Cadherin,CDH2 CDw325,cadherin-2,CD325,CDHN,CDw325,NCAD,CD325,CDHN,CDw325,NCAD,N Cadherin,CDH2
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS
translate.label.attr.clone:
B210
Clonality:
Recombinant Monoclonal
Concentration:
1 mg/ml
Extra Details:
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Host:
Rabbit
Immunogen:
Recombinant Human N-Cadherin / CD325 / CDH2 Protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Recombinant Antibodies
Flow cytometry analysis of N-Cadherin on HeLa cells. HeLa cells were harvested with (Left) or without (Right) trypsinization [please note, the epitope is sensitive to trypsin] and stained with either purified Rabbit IgG isotype control (dashed line) or Rabbit Anti-Human CD325 antibody at matching concentrations , then a FITC-conjugated second step antibody.
Immunofluorescence analysis of Human CDH2 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CDH2 Monoclonal Antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to membrane.
Western Blot with N-Cadherin / CD325 / CDH2 Monoclonal Antibody at dilution of 1:500. Lane A: Hela Whole Cell Lysate, Lysates/proteins at 30 ug per lane.
Western Blot with N-Cadherin / CD325 / CDH2 Monoclonal Antibody at dilution of 1:500. Lane A: 293T Whole Cell Lysate, Lane B: N Cadherin konckout 293T Whole Cell Lysate, Lysates/proteins at 30 ug per lane.