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Antibodies

Recombinant LRPAP1/A2MRAP Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300219P-100UL
About this Product
SKU:
AN300219P
Additional Names:
HBP;MYP;LRPAP;LRPAP1;A2MRAP;A2RAP;HBP44;MRAP;MYP23;RAP;alpha-2-MRAP;39 kDa receptor-associated protein;Alpha 2 macroglobulin receptor associated protein;Alpha 2 MRAP;Alpha-2-macroglobulin receptor-associated protein;AMRP;Lipoprotein receptor associated protein;Low density lipoprotein receptor related protein associated protein 1;Low density lipoprotein receptor-related protein-associated protein 1;Low density lipoprotein related protein associated protein 1;Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein;low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1);MGC138272
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B137
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. Mutations in this gene have been identified in individuals with myopia 20. Alternative splicing results in multiple transcript variants.
Host:
Rabbit
Immunogen:
Recombinant Human LRPAP1 protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Human LRPAP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LRPAP1 Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation analysis using 2 uL anti-LRPAP1 Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using LRPAP1 Monoclonal Antibody at a dilution of 1:200. Lane A:0.5 mg 293T Whole Cell Lysate
Western Blot with LRPAP1 / A2MRAP Monoclonal Antibody at dilution of 1:500. Lane A: U87-MG Whole Cell Lysate, Lane B: PC-3 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lysates/proteins at 30 ug per lane.