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Antibodies

Recombinant SerpinA8/Angiotensinogen/AGT Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300213P-100UL
About this Product
SKU:
AN300213P
Additional Names:
hFLT;Angiotensin 2;Angiotensin 3;AGT and SERPINA;SERPINA;Serpin A;AGT;ANHU;hFLT1;Aangiotensinogen;AGT and SERPINA8;Alpha 1 antiproteinase antitrypsin;Ang;ANGT;ANRT;AT-2;PAT;Angiotensinogen;Angiotensin-1;SERPINA8;Angiotensin-2;Angiotensin-3;Angiotensin-4;Serpin A8;Angiotensin 1-7;Angiotensin 1-10;Angiotensin I;Ang I;Angiotensin 1-8;Angiotensin II;Ang II;Angiotensin 2-8;Angiotensin III;Ang III;Angiotensin 3-8;Ang IV;Angiotensin 1-9;Angiotensin 1-5;Angiotensin 1-4;Angiotensin IV;Aangiotensinogen (serpin peptidase inhibitor clade A member 8);AI265500;Ang;AngII;Angiotensinogen (PAT);AT-II;Des-Asp[1]-angiotensin II;FLJ92595;FLJ97926;MGC105326;Pre angiotensinogen;Serine (or cysteine) proteinase inhibitor;Serpin peptidase inhibitor clade A member 8
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B131
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.Angiotensin-3 stimulates aldosterone release.Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
Host:
Rabbit
Immunogen:
Recombinant Human SerpinA8 protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human AGT expression on HepG2 cells. The cells were stained with purified anti-Human AGT, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human AGT in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human AGT Monoclonal Antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation analysis using 2 uL anti-SerpinA8 Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using SerpinA8 Monoclonal Antibody at a dilution of 1:200. Lane A:0.5 mg HepG2 Whole Cell Lysate
Western Blot with SerpinA8 / Angiotensinogen / AGT Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.