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Antibodies

Recombinant EpCAM/TROP-1/TACSTD1 Monoclonal Antibody

Product Sizes
20 ul
£189.00
AN300200P-20UL
100 ul
£402.00
AN300200P-100UL
About this Product
SKU:
AN300200P
Additional Names:
M4S;MIC;M1S;DIAR;TROP;HNPCC;GA733;TACSTD;EGP;EPCAM;DIAR5;EGP-2;EGP314;EGP40;ESA;HNPCC8;KS1/4;KSA;M4S1;MIC18;MK-1;TACSTD1;TROP1;Ep-CAM;epithelial cell adhesion molecule;GA733-2;M1S2;MK-1;KSA;EGP40;TROP1;17 1A;323/A3;35-KD glycoprotein;Adenocarcinoma associated antigen;Adenocarcinoma-associated antigen;Antigen identified by monoclonal AUA1;AUA1;CD326;CD326 antigen;Cell surface glycoprotein Trop 1;Cell surface glycoprotein Trop 2;Cell surface glycoprotein Trop-1;chromosome 4;CO 17A;CO17 1A;CO17A;EGP 2;EGP2;Ep CAM;EpCAM1;Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD);Epithelial cell surface antigen;Epithelial cellular adhesion molecule;Epithelial glycoprotein;Epithelial glycoprotein 1;Epithelial glycoprotein 314;EpithelialGlycoprotein;GA733 1;GA733 2;gastrointestinal tumor-associated antigen 2;gp4;hEGP 2;hEGP314;Human epithelial glycoprotein 2;KS 1/4 antigen;KS 1/4 antigen.KSA;Ly74;Lymphocyte antigen 74;M1S 1;Major gastrointestinal tumor associated protein GA733 2;Major gastrointestinal tumor-associated protein GA733-2;mEGP314;Membrane component;Membrane component chromosome 4 surface marker (35kD glycoprotein);MK 1;Protein 289A;surface marker;surface marker 1;TACD1;Tumor associated calcium signal transducer 1;Tumor associated calcium signal transducer 2 precursor;Tumor-associated calcium signal transducer 1
Application:
Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
B118
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Host:
Rabbit
Immunogen:
Recombinant Human EpCAM protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Flow cytometric analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab, then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells.
Immunofluorescence analysis of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM Monoclonal Antibody (1:500) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to plasma membrane.
Immunohistochemistry of paraffin-embedded human colon carcinoma using EpCAM / TROP-1 / TACSTD1 Monoclonal Antibody at dilution of 1:2500.
Immunohistochemistry of paraffin-embedded human bladder carcinoma using EpCAM / TROP-1 / TACSTD1 Monoclonal Antibody at dilution of 1:2500.
Immunoprecipitation analysis using 0.5 uL anti-EpCAM Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using EpCAM Monoclonal Antibody at a dilution of 1:5000. Lane A:0.5 mg MCF-7 Whole Cell Lysate, Lane B:0.5 mg A431 Whole Cell Lysate, Lane C:0.5 mg HepG2 Whole Cell Lysate, Lane D:0.5 mg Caco-2 Whole Cell Lysate