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Antibodies

Recombinant Latexin/LXN Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300180P-100UL
About this Product
SKU:
AN300180P
Additional Names:
ECI;Endogenous carboxypeptidase inhibitor;Latexin;Latexin protein;MUM;Protein MUM;TCI;Tissue carboxypeptidase inhibitor;LXN
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A1294
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI, and LXN, is a cytoplasm protein that belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in the heart, prostate, ovary, kidney, pancreas, and colon. Latexin/LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in the human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including the brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. Also, Latexin is implicated in Alzheimer's disease.
Host:
Rabbit
Immunogen:
Recombinant Human Latexin / LXN Protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human LXN expression on MCF-7 cells. The cells were stained with purified anti-Human LXN, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human LXN in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LXN Monoclonal Antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation analysis using 2 uL anti-LXN Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using LXN Monoclonal Antibody at a dilution of 1:200. Lane A:0.5 mg A549 Whole Cell Lysate, Lane B:0.5 mg Hela Whole Cell Lysate
Western Blot with Latexin / LXN Monoclonal Antibody at dilution of 1:500. Lane A: A549 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.