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Antibodies

Recombinant STUB1 Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300116P-100UL
About this Product
SKU:
AN300116P
Additional Names:
STUB,SCAR,UBOX,Antigen NY-CO,CLL-Associated Antigen KW,STIP1 Homology and U Box-Containing Protein,NY-CO,HSPABP,SDCCAG,STUB1,CHIP,HSPABP2,NY-CO-7,SCAR16,SDCCAG7,UBOX1,Antigen NY-CO-7,Carboxy Terminus of Hsp70-Interacting Protein,CLL-Associated Antigen KW-8,E3 Ubiquitin-Protein Ligase CHIP,STIP1 Homology and U Box-Containing Protein 1,PP1131,STIP1 homology and U-box containing protein 1
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
translate.label.attr.clone:
A1230
Clonality:
Recombinant Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2.
Host:
Rabbit
Immunogen:
Recombinant Human STUB1 protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Recombinant Antibodies
Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human STUB1 Monoclonal Antibody (dilution ratio 1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.
Immunoprecipitation analysis using 2 uL anti-STUB1 Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using STUB1 Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg Hela Whole Cell Lysate, Lane B:0.5 mg 293T Whole Cell Lysate, Lane C:0.5 mg MCF-7 Whole Cell Lysate
Western Blot with STUB1 Monoclonal Antibody at dilution of 1:500. Lane A: HELA Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: MCF7 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.