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Antibodies

Recombinant Leukotriene A4 Hydrolase/LTA4H Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300111P-100UL
About this Product
SKU:
AN300111P
Additional Names:
LTA;LKHA;LTA4H;FLJ17564;Leukotriene A(4) hydrolase;Leukotriene A4 hydrolase;Leukotriene A-4 hydrolase;LKHA4;LTA4 hydrolase;LTA-4 hydrolase;LTA4
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A1225
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H, and LTA4, is a cytoplasm protein that belongs to the peptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may, in turn, be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the pro-inflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.
Host:
Rabbit
Immunogen:
Recombinant Human Leukotriene A4 Hydrolase / LTA4H protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Human LTA4H in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LTA4H Monoclonal Antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation analysis using 0.5 uL anti-LTA4H Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using LTA4H Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg Hela Whole Cell Lysate, Lane B:0.5 mg 293T Whole Cell Lysate
Western Blot with Leukotriene A4 Hydrolase / LTA4H Monoclonal Antibody at dilution of 1:500. Lane A: Hela Whole Cell Lysate, Lane B: A549 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lysates/proteins at 30 ug per lane.