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Antibodies

Recombinant UCHL1/PGP9.5 Monoclonal Antibody

Product Sizes
20 ul
£189.00
AN300102P-20UL
100 ul
£402.00
AN300102P-100UL
About this Product
SKU:
AN300102P
Additional Names:
SPG;HEL;PARK;UCHL;Ubiquitin thioesterase L;Neuron Cytoplasmic Protein;Ubiquitin carboxyl-terminal hydrolase isozyme L;HEL-S;Uch-L;PGP;HEL-117;HEL-S-53;NDGOA;PARK5;PGP 9.5;PGP9.5;PGP95;SPG79;Uch-L1;UCHL1;Neuron Cytoplasmic Protein 9.5;Ubiquitin carboxyl-terminal hydrolase isozyme L1;Ubiquitin thioesterase L1;UCHL-1;EC:3.4.19.12;Epididymis luminal protein 117;Epididymis secretory protein Li 53;HEL 117;HEL S 53;OTTHUMP00000218137;OTTHUMP00000218139;OTTHUMP00000218140;OTTHUMP00000218141;Park 5;PGP 9.5 (PGP9.5);Protein gene product 9.5;Ubiquitin C terminal esterase L1;Ubiquitin C terminal hydrolase;Ubiquitin C terminal hydrolase L1;Ubiquitin carboxyl terminal esterase L1;Ubiquitin carboxyl terminal hydrolase isozyme L1;Ubiquitin C-terminal hydrolase L1;Ubiquitin thiolesterase;Ubiquitin thiolesterase L1;NDGOA;Neuron cytoplasmic protein 9.5;PARK5;PGP 9.5;PGP9.5;PGP95;Ubiquitin carboxyl-terminal hydrolase isozyme L1;Ubiquitin thioesterase L1;UCH-L1;UCHL1
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A1216
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Host:
Rabbit
Immunogen:
Recombinant Human UCHL1 protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of UCHL1 in DU145 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human UCHL1 Monoclonal Antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.
Immunoprecipitation analysis using 2 uL anti-UCHL1 Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using UCHL1 Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg 293T Whole Cell Lysate, Lane B:0.5 mg SH-SY5Y Whole Cell Lysate, Lane C:0.5 mg U87MG Whole Cell Lysate
Western Blot with UCHL1 / PGP9.5 Monoclonal Antibody at dilution of 1:500. Lane A: SH-SY5Y Whole Cell Lysate, Lysates/proteins at 30 ug per lane.