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Antibodies

Recombinant Phospho-AKT (Ser473) Monoclonal Antibody

Product Sizes
20 ul
£189.00
AN300086L-20UL
100 ul
£402.00
AN300086L-100UL
About this Product
SKU:
AN300086L
Additional Names:
CWS;MGC;AKT serine/threonine kinase;AKT;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA;AKT1;AKT 1;AKT serine/threonine kinase 1;AKT1_HUMAN;MGC99656;Protein kinase B;Protein Kinase B Alpha;Proto-oncogene c-Akt;RAC Alpha;RAC-alpha serine/threonine-protein kinase;RAC-PK-alpha;AKT;AKT serine/threonine kinase 1;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA
Application:
IHC-Paraffin, Western Blot
Buffer:
10 mM sodium HEPES, 150 mM NaCl, 100 ug/mL protein protectant, 50% glycerol, pH 7.5
translate.label.attr.clone:
A1196
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Host:
Rabbit
Immunogen:
A synthetic phosphopeptide corresponding to residues around Ser473 of human AKT.
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer tissue using Phospho-AKT (Ser473) Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded human breast cancer tissue using Phospho-AKT (Ser473) Monoclonal Antibody at dilution of 1:200.
Western blot analysis of extracts from serum-starved NIH-3T3 treated with PDGFA (5 ug/mL, 5 min; +), using Phospho-AKT (Ser473) Monoclonal Antibody at 1:1000, 1:50000, 1:200000 dilution.
Western blot analysis of extracts from serum-starved A431, untreated (-); treated with EGF (200 ng/mL, 15 min; +), using Phospho-AKT (Ser473) Monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower).
Western blot analysis of extracts from serum-starved NIH-3T3, untreated (-); treated with PDGFA (5 ug/mL, 5 min; +), using Phospho-AKT (Ser473) Monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower).