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Antibodies

Recombinant SMAC/Diablo Monoclonal Antibody

Product Sizes
100 ul
£402.00
AN300075P-100UL
About this Product
SKU:
AN300075P
Additional Names:
DFNA;DIABLO;DFNA64;SMAC;0610041G12Rik;DBLOH;DBOH;Diablo homolog;Diablo homolog (Drosophila);Diablo homolog mitochondrial;Diablo IAP binding mitochondrial protein;Diablo like protein;DIABLO S;Direct IAP binding protein with low pI;Direct IAP-binding protein with low pI;FLJ10537;FLJ25049;mitochondrial;Mitochondrial Smac protein;Second mitochondria derived activator of caspase;Second mitochondria-derived activator of caspase;SMAC 3;Smac protein;SMAC3
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A1185
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-61. Alternative splicing results in multiple transcript variants encoding different isoforms.
Host:
Rabbit
Immunogen:
Recombinant Human SMAC / Diablo protein
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human Diablo expression on HeLa cells. The cells were stained with purified anti-Human Diablo (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by 10339-R006 was blocked by preincubation of the purified antibody with 20ug recombinant human Diablo (Cat: PKSH031669) for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Diablo Monoclonal Antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Western Blot with SMAC / Diablo Monoclonal Antibody at dilution of 1:10000. Lane A: Hela Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: A431 Whole Cell Lysate, Lane D: MCF7 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.