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Antibodies

MCP-3/CCL7 Monoclonal Antibody

Product Sizes
100 ul
£346.00
AN200217P-100UL
About this Product
SKU:
AN200217P
Additional Names:
CCL,RP23-350G,C-C motif chemokine,Chemokine CC motif ligand,Small-inducible cytokine A,Monocyte chemotactic protein,Monocyte chemoattractant protein,MCP,CCL7,FIC,MARC,MCP-3,MCP3,NC28,SCYA6,SCYA7,C-C motif chemokine 7,Chemokine CC motif ligand 7,Monocyte chemoattractant protein 3,Monocyte chemotactic protein 3,RP23-350G1.4,Small-inducible cytokine A7
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
PBS
translate.label.attr.clone:
A1069
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q.
Host:
Mouse
Immunogen:
Recombinant Human CCL7/MCP3 protein
Isotype:
IgG1
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Human CCL7 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCL7 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope, right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human CCL7 expression on THP-1 cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human CCL7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.