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Antibodies

Ki67/MKI67 Monoclonal Antibody

Product Sizes
100 ul
£346.00
AN200153P-100UL
About this Product
SKU:
AN200153P
Additional Names:
MIB;MKI;marker of proliferation Ki;Proliferation marker protein Ki;Antigen identified by monoclonal antibody Ki;PPP1R;MKI67;KIA;MIB-;MIB-1;PPP1R105;marker of proliferation Ki-67;Ki67;Antigen identified by monoclonal antibody Ki-67;Antigen Ki67;Antigen KI-67;Proliferation marker protein Ki-67;Antigen identified by monoclonal Ki 67;Antigen identified by monoclonal Ki-67;MIB 1;Proliferation related Ki 67 antigen;Protein phosphatase 1 regulatory subunit 105;RP11-380J17.2
Application:
IHC-Paraffin, Immunocytochemistry, Immunofluorescence
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A1005
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Host:
Mouse
Immunogen:
A synthetic peptide corresponding to the center region of the Human Ki67/MKI67
Isotype:
IgG2b
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human small intestine using Ki67/MKI67 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human liver using Ki67/MKI67 Monoclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of MKI67 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human MKI67 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Nucleus.