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Antibodies

RhoA Monoclonal Antibody

Product Sizes
100 ul
£346.00
AN200131P-100UL
About this Product
SKU:
AN200131P
Additional Names:
ARH,RHOH,ARH12,ARHA,RHO12,RHOH12,Rho,RHOA,EDFAOB,ARH12,EDFAOB,RHO12,RHOH12,Aplysia ras related homolog 12,H 12,H12,Oncogene RHO H12,ras homolog family member A,Ras homolog gene family member A,Rho A,Rho cDNA clone 12,RHO H12,Small GTP binding protein Rho A,Transforming protein Rho A,Transforming protein RhoA
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
PBS
translate.label.attr.clone:
A979
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.
Host:
Mouse
Immunogen:
Recombinant Human RhoA protein
Isotype:
IgG2a
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human RHOA expression in HeLa cells. The cells were and stained with Purified Mouse anti-RhoA Monoclonal Antibody, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human RHOA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human RHOA Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.