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Antibodies

Beta-2 microglobulin/B2M Monoclonal Antibody

Product Sizes
20 ul
£167.00
AN200123P-20UL
100 ul
£346.00
AN200123P-100UL
About this Product
SKU:
AN200123P
Additional Names:
IMD,Beta-2-microglobulin form pI,CDABP,B2M,IMD43,B2MG,Beta 2 microglobin,Beta 2 microglobulin,Beta 2 microglobulin precursor,Beta chain of mhc class 1 proteins,Beta chain of MHC class I molecules,Beta-2-Microglobulin,Beta-2-microglobulin form pI 5.3,B Beta2M,B Beta2-Microglobulin,CDABP0092,HDCMA22P
Application:
Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS
translate.label.attr.clone:
A971
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Host:
Mouse
Immunogen:
Recombinant Human Beta-2 microglobulin/B2M Protein
Isotype:
IgG1
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human B2M / Beta-2-microglobulin expression on human whole blood lymphocytes. Cells were stained with purified anti-Human B2M / Beta-2-microglobulin, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Immunofluorescence analysis of B2M in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human B2M Monoclonal Antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue).Positive staining was localized to Cytoplasm and cell membrane.
Immunohistochemistry of paraffin-embedded human ovarian cancer using Beta-2 microglobulin/B2M Monoclonal Antibody at dilution of 1:60.
Immunohistochemistry of paraffin-embedded human skin using Beta-2 microglobulin/B2M Monoclonal Antibody at dilution of 1:60.
Western Blot with Beta-2 microglobulin/B2M Monoclonal Antibody at dilution of 1:500. Lane A: A431 Whole Cell Lysate, Lane B: U937 Whole Cell Lysate, Lane C: Raji Whole Cell Lysate, Lane D: HeLa Whole Cell Lysate, Lysates/proteins at 30 ug per lane.