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Antibodies

BID Monoclonal Antibody

Product Sizes
20 ul
£167.00
AN200104P-20UL
100 ul
£346.00
AN200104P-100UL
About this Product
SKU:
AN200104P
Additional Names:
BID,FP497,BH3-Interacting Domain Death Agonist,p22 BID
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS
translate.label.attr.clone:
A952
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP5 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Host:
Mouse
Immunogen:
Recombinant Human BID protein
Isotype:
IgG1
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Human BID expression in Jurkat cells. The cells were and stained with Purified Mouse anti-BID (10486-MM10), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human BID in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human BID Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Western Blot with BID Monoclonal Antibody at dilution of 1:500. Lane A: Jurkat Whole Cell Lysate, Lysates/proteins at 30 ug per lane.