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Antibodies

SAE1 Monoclonal Antibody

Product Sizes
100 ul
£346.00
AN200081P-100UL
About this Product
SKU:
AN200081P
Additional Names:
AOS,SUA,SAE,AOS1,SUA1,UBLE1A,SAE1,Activator of SUMO1,HSPC140,Sentrin/SUMO activating protein AOS1,SUMO 1 activating enzyme E1 N subunit,SUMO 1 activating enzyme subunit 1,SUMO-activating enzyme subunit 1,Ubiquitin like protein SUMO1 activating enzyme,Ubiquitin-like 1-activating enzyme E1A,UBL E1A
Application:
Immunocytochemistry, Immunofluorescence
Buffer:
PBS
translate.label.attr.clone:
A929
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
SAE1 belongs to the ubiquitin-activating E1 family. It is a heterodimer that acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 also regulates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.
Host:
Mouse
Immunogen:
Recombinant Human SAE1 protein
Isotype:
IgG2b
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of SAE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Y SAE1 Monoclonal Antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue).Positive staining was localized to Nucleus.