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Antibodies

ENO3/beta-enolase Monoclonal Antibody

Product Sizes
100 ul
£346.00
AN200045P-100UL
About this Product
SKU:
AN200045P
Additional Names:
GSD,ENO,enolase,ENO3,GSD13,MSE,enolase 3,2 phospho D glycerate hydrol yase,2 phospho D glycerate hydrolyase,2-phospho-D-glycerate hydro-lyase,Beta enolase,Beta-enolase,ENOB,Enolase 3 (beta muscle),Enolase3,Muscle specific enolase,Muscle-specific enolase,Skeletal muscle enolase
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS
translate.label.attr.clone:
A889
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.
Host:
Mouse
Immunogen:
Recombinant Human ENO3 / beta-enolase Protein
Isotype:
IgG2a
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human ENO3 expression on HeLa cells. The cells were stained with purified anti-Human ENO3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of ENO3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human ENO3 Monoclonal Antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor?594-conjugated Goat Anti-mouse IgG secondary antibody (red). Positive staining was localized to Cytoplasm.
Western Blot with ENO3 / beta-enolase Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lane C: MOLT4 Whole Cell Lysate, Lane D: Raji Whole Cell Lysate, Lysates/proteins at 30 ug per lane.