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Antibodies

IL-1RA/IL1RN Monoclonal Antibody

Product Sizes
100 ul
£346.00
AN200036P-100UL
About this Product
SKU:
AN200036P
Additional Names:
IL1F;MVCD;IL1RN;DIRA;ICIL-1RA;IL-1RN;IL-1ra;IL-1ra3;IL1F3;IL1RA;IRAP;MVCD4;Anakinra;IL1 inhibitor;Interleukin-1 receptor antagonist protein;F630041P17Rik;ICIL 1RA;ICIL1RA;IL1RN (IL1F3);Interleukin 1 receptor antagonist;Intracellular IL 1 receptor antagonist type II;Intracellular interleukin 1 receptor antagonist (icIL 1ra);MGC10430;Type II interleukin 1 receptor antagonist
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A880
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.
Host:
Mouse
Immunogen:
Recombinant Human IL-1RA protein
Isotype:
IgG2b
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human IL1RA expression on A431 cells. Cells were stained with purified anti-Human IL1RA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human IL1Ra in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human IL1Ra Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.