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Antibodies

14-3-3 beta/YWHAB Monoclonal Antibody

Product Sizes
100 ul
£346.00
AN200031P-100UL
About this Product
SKU:
AN200031P
Additional Names:
Protein kinase C inhibitor protein,HEL-S,Protein,YWHAB,GW128,HEL-S-1,HS1,KCIP-1,YWHAA,14 3 3 alpha,14 3 3 protein beta/alpha,14-3-3 protein beta/alpha,1433B,Brain protein 14 3 3 beta isoform,HS 1,KCIP1,N-terminally processed,Protein 1054,Protein kinase C inhibitor protein 1
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
PBS
translate.label.attr.clone:
A875
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC22 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
Host:
Mouse
Immunogen:
Recombinant Human 14-3-3 beta / YWHAB protein
Isotype:
IgG2b
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human YWHAB expression on Molt-4 cells. The cells were stained with purified anti-Human YWHAB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human YWHAB in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human YWHAB Monoclonal Antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.