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Antibodies

HIGD1A Polyclonal Antibody

Product Sizes
100 ul
£346.00
AN100007P-100UL
About this Product
SKU:
AN100007P
Additional Names:
HIG,HSPC,HIGD1A,HIG1,RCF1a,HSPC010
Application:
IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Proposed subunit of cytochrome c oxidase (COX, complex IV, which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes.
Host:
Rabbit
Immunogen:
E. coli-derived Human HIGD1A fragment
Isotype:
IgG
Purification:
Affinity Purified; Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Polyclonal Antibody
Immunofluorescence analysis of HIGD1A in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human HIGD1A polyclonal antibody (dilution ratio 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry of paraffin-embedded human rectum using HIGD1A Polyclonal Antibody at dilution of 1:100.
Immunoprecipitation analysis using 4 uL anti-HIGD1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Western blot was performed from the immunoprecipitate using HIGD1A rabbit polyclonal antibody at a dilution of 1:100. Lane A:0.5 mg A431 Whole Cell Lysate, Lane B:0.5 mg HeLa Whole Cell Lysate, Lane C:0.5 mg HepG2 Whole Cell Lysate, Lane D:0.5 mg 293 Whole Cell Lysate
Western Blot with HIGD1A Polyclonal Antibody at dilution of 1:500. Lane A: A431 Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lane C: HepG2 Whole Cell Lysate, Lane D: 293 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.