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Antibodies

PAK1 Antibody Polyclonal Antibody

Product Sizes
100 ul
£346.00
AN100004P-100UL
About this Product
SKU:
AN100004P
Additional Names:
PAK,Pak1
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
P21-activated kinase-1 (PAK1) is an enzyme associated with multiple metabolic networks and different types of cancers.PAK1 (RAC/CDC42-activated kinase 1) is the major oncogenic/ageing kinase, and its dysfunction extends the healthy lifespan of C. elegans by activating HSP16 gene. The p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1) expression appears to be predictive of prognosis in various solid tumors. PAK1 expression may be a predictive marker of overall survival and disease-specific survival in patients with solid tumors.p21-Activated kinase 1 (PAK1) has attracted much attention as a potential therapeutic target due to its central role in many oncogenic signaling pathways, its frequent dysregulation in cancers and neurological disorders, and its tractability as a target for small-molecule inhibition.
Host:
Rabbit
Immunogen:
A synthetic peptide corresponding to the C-terminus of the Rat PAK1
Isotype:
IgG
Purification:
Affinity Purified; Protein A Purified
Reactivities:
Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Polyclonal Antibody
Immunofluorescence analysis of PAK1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-rat PAK1 polyclonal antibody (dilution ratio: 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm.
Immunoprecipitation analysis using 1 uL anti-PAK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using PAK1 rabbit polyclonal antibody at a dilution of 1:100. Lane A:0.5 mg 293T Whole Cell Lysate, Lane B:0.5 mg NIH-3T3 Whole Cell Lysate
Western Blot with PAK1 Antibody Polyclonal Antibody at dilution of 1:500. Lane A: HeLa Whole Cell Lysate, Lane B: A431 Whole Cell Lysate, Lane C: 293 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.