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Antibodies

Purified Anti-Mouse CD45RO/B220 Antibody[RA3-6B2], Functional Grade

Product Sizes
100 ug
£89.00
AN004280-100UG
1 mg
£171.00
AN004280-1MG
5 mg
£490.00
AN004280-5MG
25 mg
£1515.00
AN004280-25MG
50 mg
£2247.00
AN004280-50MG
100 mg
£3211.00
AN004280-100MG
About this Product
SKU:
AN004280
Application:
Activate/Stimulate, Flow Cytometry
Buffer:
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
translate.label.attr.clone:
RA3-6B2
Clonality:
Monoclonal
Concentration:
>= 1 mg/ml
Extra Details:
CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker, however, CD19 may be more appropriate for B cell specificity. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
Host:
Rat
Immunogen:
Recombinant Mouse B220 protein
Isotype:
IgG2a, kappa
Purity:
>98%
Purification:
protein a/g purified
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C Avoid freeze/thaw cycles., -20[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
C57/BL6 Mouse splenocytes were stained with 0.2 ug Purified Anti-Mouse CD45RO/B220 Antibody[RA3-6B2], Functional Grade (Right) and 0.2 ug Rat IgG2a, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Rat IgG Secondary Antibody, then anti-Mouse CD19 PE-conjugated Monoclonal Antibody.
Human peripheral blood lymphocytes were stained with 0.2 ug Purified Anti-Human CD279 Antibody[EH12.2H7] (Right) and 0.2 ug Mouse IgG1, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD3 PE-conjugated Monoclonal Antibody.
Rat splenocytes were stained with 0.2 ug AF/LE Purified Anti-Rat CD90 Antibody[OX-7] (Right) and 0.2 ug Mouse IgG1, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Rat CD3 PE-conjugated Monoclonal Antibody.
Human peripheral blood lymphocytes were stained with 0.2 ug AF/LE Purified Anti-Human CD3 Antibody[UCHT1] (Right) and 0.2 ug Mouse IgG1, K Kappa Isotype Control (Left), followed by PE-conjugated Goat Anti-Mouse IgG Secondary Antibody.
C57/BL6 Mouse splenocytes were stained with 0.2 ug Purified Anti-Mouse H-2 Antibody[M1/42] (Right) and 0.2 ug Rat IgG2a, K Kappa Isotype Control (Left), followed by FITC-conjugated Goat Anti-Rat IgG Secondary Antibody.