PI(4,5)P2 PIP Beads
Product Sizes
100 ul
£288.00
ECH-P-B045A-100UL
1 ml
£1179.00
ECH-P-B045A-1ML
About this Product
- SKU:
- ECH-P-B045A
- Extra Details:
- PI(4,5)P2 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(4,5)P2 per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase. \n \n \n \nStorage \n4 °C - do not freeze \nApplications \nPIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis. \nFeatured in References \n1) Gupta, S., J. C. Fanzo, et al. (2003). "T cell receptor engagement leads to the recruitment of IBP, a novel guanine nucleotide exchange factor, to the immunological synapse." J Biol Chem 278(44): 43541-9. \n2) Kawahara, T. and J. D. Lambeth (2008). "Phosphatidylinositol (4,5)-bisphosphate modulates Nox5 localization via an N-terminal polybasic region." Mol Biol Cell 19(10): 4020-31. \n3) Kim, S. K., H. Kim, et al. (2011). "Phosphatidylinositol phosphates directly bind to neurofilament light chain (NF-L) for the regulation of NF-L self assembly." Exp Mol Med 43(3): 153-60. \n4) Lewis, A. E., et al. (2011). "Identification of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins by neomycin extraction." Mol Cell Proteomics 10(2): M110 003376. \n5) Ulicna, L., et al. (2018). "PIP2 epigenetically represses rRNA genes transcription interacting with PHF8." Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1863(3): 266-275. \n6) Huang, D., et al. (2019). "Hypoxia induces actin cytoskeleton remodeling by regulating the binding of CAPZA1 to F-actin via PIP2 to drive EMT in hepatocellular carcinoma." Cancer Letters 448(28): 117-127. \n \nBeing bio-reactive blog: "Lipid-Ligand interaction tools: lipids are not scary intermediates anymore" \n \n \n \nDocuments \nTechnical Data Sheet, Echelon
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 2-8[o]C Do not freeze.
- Supplier:
- Echelon Biosciences
- Type:
- Microspheres: Protein Coated
