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Microspheres

PI(3,5)P2 PIP Beads

Product Sizes
100 ul
£288.00
ECH-P-B035A-100UL
1 ml
£1179.00
ECH-P-B035A-1ML
About this Product
SKU:
ECH-P-B035A
Extra Details:
PI(3,5)P2 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(3,5)P2 per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase. \n \n \n \nStorage \n4 °C - do not freeze \nApplications \nPIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis. \nFeatured in Publications \n1) Dong, X.-p., D. Shen, et al. (2010). "PI(3,5)P2 controls membrane trafficking by direct activation of mucolipin Ca2+ release channels in the endolysosome." Nat Commun 1(4): 38. \n2) Galvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039. \n3) Li, X., et al. (2013). "Genetically encoded fluorescent probe to visualize intracellular phosphatidylinositol 3,5-bisphosphate localization and dynamics." Proc Natl Acad Sci U S A 110(52): 21165-21170. \n \nBeing bio-reactive blog: "Lipid-Ligand interaction tools: lipids are not scary intermediates anymore" \n \n \n \nDocuments \nTechnical Data Sheet, Echelon
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C Do not freeze.
Supplier:
Echelon Biosciences
Type:
Microspheres: Protein Coated