Anti-PEth Autologous IgG ELISA
Product Sizes
1 kit
£1188.00
ECH-K-5000A-1KIT
About this Product
- SKU:
- ECH-K-5000A
- Extra Details:
- The Anti-PEth Autologous IgG ELISA is a 96-well ELISA Assay for Detection of Autologous PEth IgG antibodies. \n \nAssay Sensitivity: LOD 80 ng/mL \nAssay Incubation Time: 3.5 hours \nSample Type: Serum and plasma samples \nAssay Range: 80 ng/mL to 45 ug/mL \nSample Considerations: Run Samples at 1:125 dilution in provided sample buffer. Serum demonstrates a 25 to 30% stronger signal than plasma. \nSpecies of Sample: Human \nSample Volume: 10 uL minimum \n \n \n \nProduct Description \nThe Human Anti-PEth Autologous IgG ELISA is a direct assay in which the signal is proportional to the amount of autologous anti-PEth IgG contained in the sample. The serum (or plasma) sample is added to a PEth-coated plate for binding. A peroxidase-linked secondary detector and colorimetric substrate are used to detect the amount of anti-PEth antibody bound to the plate. The colorimetric signal is read at absorbance 450 nm and is directly proportional to the amount of autologous anti-PEth IgG in the sample. \n \nEthanol (EtOH) has multiple effects on humans and other animals. Alcohol addiction and organ damage are active areas of research with a need for research reagents and tools.1 Phosphatidylethanol (PEth) is a direct metabolite of EtOH and has been described as a long-lived biomarker for alcohol ingestion.2, 3 It is possible that PEth is involved in the neurological4, protective5, and harmful6 effects of alcohol, but the mechanisms are still being explored. \n \nAutologous anti-PEth antibodies are found in lower abundance in serum from heavier drinkers. This inverse relationship between anti-PEth IgG levels and alcohol ingestion was first reported, by Nissinen et. al.7 Evaluation of serum samples of varying consumption habits has demonstrated a high degree of correlation with ingested alcohol. Measuring these autologous anti-PEth IgG levels may serve as an effective biomarker in monitoring alcohol use. \nReferences \n1. Freeman, W. M.; Vrana, K. E. Future prospects for biomarkers of alcohol consumption and alcohol-induced disorders. Alcohol Clin Exp Res 2010, 34, 946-54. \n2. Viel, G.; et al. Phosphatidylethanol in blood as a marker of chronic alcohol use: a systematic review and meta-analysis. Int J Mol Sci 2012, 13, 14788-812. \n3. Wurst, F. M.; et al. Phosphatidylethanol: normalization during detoxification, gender aspects and correlation with other biomarkers and self-reports. Addict Biol 2010, 15, 88-95. \n4. Esteban-Pretel, G.; et al. Polyphosphoinositide metabolism and Golgi complex morphology in hippocampal neurons in primary culture is altered by chronic ethanol exposure. Alcohol Alcohol 2012, 48, 15-27. \n \n \n \nDocuments \nTechnical Data Sheet \n \nTDS _K-5000a_Rev2a, Echelon
- Immunogen:
- Anti-PEth Autologous IgG ELISA
- Shipping Conditions:
- Blue Ice
- Supplier:
- Echelon Biosciences
- Type:
- Assay Kits: ELISAs
