Sphingosine Kinase Activity Assay
Product Sizes
1 kit
£781.00
ECH-K-3500-1KIT
About this Product
- SKU:
- ECH-K-3500
- Extra Details:
- The Sphingosine Kinase Activity Assay is a luminescent assay that measures sphingosine kinase activity by detecting the decrease of ATP. \n \nSample Type: purified enzyme, cell lysate/homogenate \nSample Volume: 10 uL / sample (duplicate points) \nAssay Incubation Time: 30 minutes to 2 hours \n \n \n \nProduct Background \n \nThe Sphingosine Kinase Activity Assay is prepared with the addition of sphingosine kinase, sphingosine (substrate) in reaction buffer, and initiated by the addition of ATP. The reaction is stopped by addition of the ATP detector at the chosen reaction time. The luminescent signal is inversely correlated with the kinase activity. The sphingosine kinase activity assay was tested with Echelon's SPHK1 and SPHK2. \n \nSphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). SPHKs have been implicated in proliferation, survival, migration and regulation of Ca2+ homoeostasis, development and regulation of the cardiovascular and nervous systems, inflammation, immunity, and cancer growth. There are two currently known SPHK isoforms, SPHK1 and SPHK2. It is believed that SPHK1 and SPHK2 have opposite functions, promoting cell growth and apoptosis respectively. \n \nFeatured in Publications \n \n1. Li, QF., Zhu, HY. et al. "Prokineticin-1/endocrine gland-derived vascular endothelial growth factor is a survival factor for human multiple myeloma cells." Leuk Lymphoma 51:10 \n2. Cho, SY., Lee, HJ. et al. "Sphingosine kinase 1 pathway is involved in melatonin-induced HIF-1 inactivation in hypoxic PC-3 prostate cancer cells." J Pineal Res 51:1 \n3.Guan, H., Song, L. et al. "Sphingosine kinase 1 regulates the Akt/FOXO3a/Bim pathway and contributes to apoptosis resistance in glioma cells." PLoS One 6: 5 \n \nProduct Keywords: Sphingosine kinase, SPHK, SK, Sphingosine kinase-1, SPHK1 enzyme, SK1, Sphingosine kinase type 2, SPHK2, SK2 \n \n, Echelon
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- Echelon Biosciences
- Type:
- Assay Kits: Detection
