Neutral Sphingomyelinase Activity Assay Kit
Product Sizes
1 kit
ECH-K-1800-1KIT
About this Product
- SKU:
- ECH-K-1800
- Extra Details:
- The Neutral Sphingomyelinase Activity Assay kit is an enzyme coupled assay that measures sphingomyelinase activity in biological samples through the downstream production of choline.Assay Incubation time: 2-4 hoursAssay Range: 5 mU/mL - 0.078 mU/mLSample Type: tissue homogenates cell lysates serum saliva and urineProduct BackgroundThe Neutral Sphingomyelinase Activity Assay kit can detect sphingomyelinase at concentrations as small as 78 uU/mL. The kit provides all reagents to measure the Neutral Sphingomyelinase (nSMase) activity of 40 samples ran in duplicate.In this enzyme coupled assay N-SMase catalyzes the hydrolysis of sphingomyelin into phosphorylcholine and ceramide. Alkaline phosphatase then catalyzes phosphorylcholine to choline. Choline is them oxidized by Choline oxidase to form hydrogen peroxide. Finally DAOS plus 4-Aminoantipyrine (4 AAP) in the presence of hydrogen peroxide and peroxidase results in the oxidative coupling of DAOS and 4 AAP to form a blue chromogen that is detected by measuring the absorbance of light at 595 nm. Powered by Bioz See more details on Bioz
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- Echelon Biosciences
- Type:
- Assay Kits: Detection
