Neutral Sphingomyelinase Activity Assay Kit
Product Sizes
1 kit
ECH-K-1800-1KIT
About this Product
- SKU:
- ECH-K-1800
- Extra Details:
- The Neutral Sphingomyelinase Activity Assay kit is an enzyme coupled assay that measures sphingomyelinase activity in biological samples through the downstream production of choline. \n \nAssay Incubation time: 2-4 hours \nAssay Range: 5 mU/mL - 0.078 mU/mL \nSample Type: tissue homogenates, cell lysates, serum, saliva, and urine \n \n \n \nProduct Background \nThe Neutral Sphingomyelinase Activity Assay kit can detect sphingomyelinase at concentrations as small as 78 uU/mL. The kit provides all reagents to measure the Neutral Sphingomyelinase (nSMase) activity of 40 samples ran in duplicate. \n \nIn this enzyme coupled assay, N-SMase catalyzes the hydrolysis of sphingomyelin into phosphorylcholine and ceramide. Alkaline phosphatase then catalyzes phosphorylcholine to choline. Choline is them oxidized by Choline oxidase to form hydrogen peroxide. Finally, DAOS plus 4-Aminoantipyrine (4 AAP) in the presence of hydrogen peroxide and peroxidase results in the oxidative coupling of DAOS and 4 AAP to form a blue chromogen that is detected by measuring the absorbance of light at 595 nm. \nFeatured in Publications \n1. Qin, J., E. Berdyshev, et al. (2012). "Neutral sphingomyelinase 2 deficiency increases hyaluronan synthesis by up-regulation of hyaluronan Synthase 2 through decreased ceramide production and activation of Akt." J Biol Chem. \n2. Liu, H., et al. (2013). "Fatty acid synthase causes drug resistance by inhibiting TNF-alpha and ceramide production." J Lipid Res 54(3): 776-785. \n3. Miyauchi, S., et al. (2016). "Sphingomyelin Phosphodiesterase 3 Enhances Cytodifferentiation of Periodontal Ligament Cells." Journal of Dental Research 96(3): 339-346. \n4. Sackmann, V., et al. (2019). "Inhibition of nSMase2 Reduces the Transfer of Oligomeric A Alpha-Synuclein Irrespective of Hypoxia." Frontiers in Molecular Neuroscience 12(200) DOI: 10.3389/fnmol.2019.00200 \n5. Wu, X., et al. (2019). "Inverse Correlation Between Plasma Sphingosine-1-Phosphate and Ceramide Concentrations in Septic Patients and Their Utility in Predicting Mortality." Shock (Augusta, Ga.) 51(6): 718-724. \n \n \n \nDocuments \nTechnical Data Sheet, Echelon
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- Echelon Biosciences
- Type:
- Assay Kits: Detection
