PI3-Kinase Activity AlphaScreen Assay
Product Sizes
1 kit
ECH-K-1300-1KIT
About this Product
- SKU:
- ECH-K-1300
- Extra Details:
- The PI3-Kinase Activity AlphaScreen® Assay is a sensitive and robust method for the quantification of PI(3 4 5)P3 (PIP3) the end product of Class I PI3K reactions. This high-throughput (384-well) assay can be used for in vitro determination of PI3K activity in cancer tissues and cell lines and compound screening for drug discovery.Assay Range: 0.46 NM to 1 000 nMSample Type: purified enzyme inhibitor screenSample Volume: 2.5 uL PI3-K inhibitorProduct BackgroundWhen PIP3 is generated via phosphorylation of PI(4 5)P2 (PIP2) by PI3-Kinase the products of the enzymatic reaction compete with biotinylated I(1 3 4 5)P4 for the interaction with the PIP3 detector protein. The interaction of biotinylated-I(1 3 4 5)P4 and the PIP3 detector protein brings both Acceptor and Donor beads together producing a cascade of chemical reactions and leading to the AlphaScreen® signal. In the absence of this interaction proximity of the Donor and Acceptor beads is decreased producing a loss of luminescent signal which is inversely related to enzyme activity. AlphaScreen® technology is based on the emission of light (520-620nm) by Acceptor beads activated by the proximity of Donor beads.Echelon has multiple formats available for Class III PI3-K. Please see the PI3-K Assay Comparison Guide below for more information.Things to consider: This product is intended for use with the Amplified Luminescent Proximity Homogenous Assay (AlphaScreen®) technology and requires the researcher to obtain donor and acceptor beads from the AlphaScreen® GST (Glutathione-S-Transferase) Detection Kit which must be purchased separately from PerkinElmer Life Sciences. A microplate reader with AlphaScreen® capabilities is also required.Product Keywords: PI3-Kinase PI3K Phosphatidylinositol 3-kinases PI 3-kinases PI3Ks K-1000 PIP3 PI(345)P3 PtdIns(3 4 5)P3 Phosphatidylinositol (3 4 5)-triphosphateBulk Discounts: Bulk discounts are available please email echelon@echelon-inc.com for details Powered by Bioz See more details on BiozNote to PurchaserEchelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. This kit and all non-radioactive competitive assays for determining phosphoinositide-3-kinase (PI3-K) activity are covered by Echelon Biosciences Inc. U.S. Patent 7 067 269. The purchase of this product includes a limited non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email busdev@echelon-inc.com.
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- Echelon Biosciences
- Type:
- Assay Kits: Detection
