PI3-Kinase Activity Fluorescence Polarization Assay
Product Sizes
1 kit
ECH-K-1100-1KIT
About this Product
- SKU:
- ECH-K-1100
- Extra Details:
- The PI3-Kinase Activity Fluorescence Polarization Assay determines PI3-K activity through quantification of its product PI(3 4 5)P3 (PIP3).Sample Type: in vitro determination of PI3-K activity in cancer tissues and cell lines and compound screening for drug discoverySample Volume: 2.5-5.0 uL enzyme / inhibitorAssay Range: 0.0625 uM to 4.0 uMProduct BackgroundThe PI3-Kinase Activity Fluorescence Polarization Assay measures PIP3. Briefly once PI3-K reaction are complete the PIP3 detector protein and the fluorescent probe are added. Polarization (mP) values decrease as probe binding to the PIP3 detector is displaced by the PIP3 produced by enzymatic activity.Class I Phosphoinositide 3-kinases (PI3-K) are ubiquitously expressed lipid kinases which phosphorylate PI(4 5)P2 (PIP2) at the 3-hydroxyl of the inositol ring producing PIP3 has shown to be an important lipid second messenger with key roles in fundamental cellular responses such as proliferation and survival. Due to these interesting biological roles PI3-K and PIP3 have become attractive targets for research and drug development in many diseases including inflammation and cancer.Echelon has multiple formats available for Class III PI3-K. Please see the PI3-K Assay Comparison Guide below for more information.Product Keywords: PI3-Kinase PI3K Phosphatidylinositol 3-kinases PI 3-kinases PI3Ks K-1000Bulk Discounts: Bulk discounts are available please email echelon@echelon-inc.com for details Powered by Bioz See more details on BiozNotice to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. This kit and all non-radioactive competitive assays for determining phosphoinositide-3-kinase (PI3-K) activity are covered by Echelon Biosciences Inc. U.S. Patent 7 067 269. The purchase of this product includes a limited non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email busdev@echelon-inc.com.
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- Echelon Biosciences
- Type:
- Assay Kits: Detection
