Ac-Val-Asp-Val-Ala-Asp-AMC (Caspase 2 (ICH-1) Substrate)
Product Sizes
5 mg
£275.00
ECH-860-48-5MG
25 mg
£816.00
ECH-860-48-25MG
About this Product
- SKU:
- ECH-860-48
- Extra Details:
- Molecular Weight: 716.3 \nSalt Form: N/A \nPurity: >96% \nSequence (3-letter): Ac-Val-Asp-Val-Ala-Asp-AMC \nSequence (1-letter): Ac-VDVAD-AMC \nStorage: -20 ?C or below \n \nAc-Val-Asp-Val-Ala-Asp-AMC is a fluorogenic substrate for Caspase 2. Caspase 2, part of the ICH-1 sub-family, is a cysteine protease with a poorly defined role in apoptosis. It associates with several apoptotic proteins such as death effector filament-forming Ced-4-like apoptosis protein (DEFCAP), RIP-associated Ich-1/Ced-3-homologue protein with a death domain (RAIDD), and apoptosis repressor with caspase recruitment domain (ARC). Caspase 2 can be activated by the PIDDosome formed by the association of PIDD1 and CRADD. Recent work demonstrates that Caspase-2 resides in the mitochondria and is essential for stress-induced apoptosis. Caspase-2 cleaves at an Asp residue with a preferred sequence of Val-Asp-Val-Ala-Asp-X. \n \nActivity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm. \nReferences \n1) Luca L. Fava, Florian J. Bock, Stephan Geley, Andreas Villunger ?Caspase-2 at a Glance? Journal of Cell Science 2012 125: 5911-5915; doi: 10.1242/jcs.115105> \n2) Lisa Bouchier-Hayes ?The role of caspase-2 in stress-induced apoptosis? J Cell Mol Med. 2010 Jun; 14(6a): 1212?1224. \n3) M Lopez-Cruzan, R Sharma, M Tiwari, S Karbach, D Holstein, C R Martin, J D Lechleiter & B Herman ?Caspase-2 resides in the mitochondria and mediates apoptosis directly from the mitochondrial compartment? Cell Death Discovery vol 2, Article number: 16005 (2016)., Echelon
- Molecular Weight:
- 716.3
- Sequence:
- Ac-Val-Asp-Val-Ala-Asp-AMC \n
- Shipping Conditions:
- Ambient
- Storage Conditions:
- -20[o]C
- Supplier:
- Echelon Biosciences
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Peptides
- Manufacturer's Data Sheet:https://www.echelon-inc.com/product/ac-val-asp-val-ala-asp-amc-caspase-2-ich-1-substrate-fluorogenic/
