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MDCK-SIAT1 Cells - Canine cell lines

Product Sizes
1 each
£607.00
602281-1EACH
About this Product
SKU:
602281
CE/IVD:
RUO
Extra Details:
The MDCK-SIAT1 cell line is a modified version of the Madin-Darby Canine Kidney (MDCK) cells, engineered to express higher levels of human 2,6-sialyltransferase (SIAT1). This enzyme is responsible for the addition of sialic acid in an alpha-2,6 linkage to galactose on glycoproteins and glycolipids. The modification was performed to increase the expression of alpha-2,6-linked sialic acids, which are the primary receptors for human influenza viruses. This enhancement is critical as it makes the MDCK-SIAT1 cells more similar to the human airway epithelium, which naturally has a high concentration of these receptors. As a result, these cells offer a more physiologically relevant model for studying human influenza viruses and their interactions with potential antiviral compounds. One of the significant applications of MDCK-SIAT1 cells is in the assessment of influenza virus sensitivity to neuraminidase inhibitors (NAIs), such as oseltamivir. Due to the increased presence of alpha-2,6-linked sialic acids, the MDCK-SIAT1 cells demonstrate improved sensitivity to NAIs compared to unmodified MDCK cells. This makes them an excellent tool for detecting resistance to these inhibitors, especially in low-passage-number clinical isolates of human influenza viruses. The MDCK-SIAT1 cell line allows for more accurate in vitro studies of drug efficacy and viral receptor interactions, providing valuable insights into the development of antiviral therapies and resistance mechanisms.
Shipping Conditions:
Dry Ice
Storage Conditions:
Liquid N2, -70[o]C Avoid freeze/thaw cycles.
Supplier:
Cytion
Type:
Cells: Cell Lines