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Antibodies

GRB2 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA981267A0HU-50UL
100 ul
£392.00
CSB-RA981267A0HU-100UL
About this Product
SKU:
CSB-RA981267A0HU
Additional Names:
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2), GRB2, ASH
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
6E1
Clonality:
Recombinant Monoclonal
Extra Details:
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Immunogen:
A synthesized peptide derived from human GRB2
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA981267A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate All lanes: GRB2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26, 21 kDa Observed band size: 28 kDa