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Antibodies

CD274 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA977797A0HU-50UL
100 ul
£392.00
CSB-RA977797A0HU-100UL
About this Product
SKU:
CSB-RA977797A0HU
Additional Names:
Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274), CD274, B7H1 PDCD1L1 PDCD1LG1 PDL1
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
10D4
Clonality:
Recombinant Monoclonal
Extra Details:
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Immunogen:
A synthesized peptide derived from human PD-L1 (CD274)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA977797A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate All lanes: CD274 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 21, 21 kDa Observed band size: 45 kDa