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Antibodies

ILK Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA963626A0HU-50UL
100 ul
£392.00
CSB-RA963626A0HU-100UL
About this Product
SKU:
CSB-RA963626A0HU
Additional Names:
Integrin-linked protein kinase (EC 2.7.11.1) (59 kDa serine/threonine-protein kinase) (ILK-1) (ILK-2) (p59ILK), ILK, ILK1 ILK2
Application:
ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
6A5
Clonality:
Recombinant Monoclonal
Extra Details:
Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715).
Immunogen:
A synthesized peptide derived from human ILK
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of MCF7 Cells with CSB-RA963626A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating ILK in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA963626A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA963626A0HU(2ug)+ Hela whole cell lysate(500ug) Lane 3: Hela whole cell lysate (10ug)
Western Blot Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue All lanes: ILK antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 45, 37 kDa Observed band size: 51 kDa