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Antibodies

CD8A Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA934881A0HU-50UL
100 ul
£392.00
CSB-RA934881A0HU-100UL
About this Product
SKU:
CSB-RA934881A0HU
Additional Names:
T-cell surface glycoprotein CD8 alpha chain (T-lymphocyte differentiation antigen T8/Leu-2) (CD antigen CD8a), CD8A, MAL
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
2F2
Clonality:
Recombinant Monoclonal
Extra Details:
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Immunogen:
A synthesized peptide derived from human CD8
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA934881A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA934881A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating CD8A in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA934881A0HU in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2:CSB-RA934881A0HU(3ug)+ HepG2 whole cell lysate(500ug) Lane 3: HepG2 whole cell lysate (10ug)
Western Blot Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: CD8A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26, 22, 31 kDa Observed band size: 31 kDa