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Antibodies

NPR2 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA926985A0HU-50UL
100 ul
£392.00
CSB-RA926985A0HU-100UL
About this Product
SKU:
CSB-RA926985A0HU
Additional Names:
Atrial natriuretic peptide receptor 2 (EC 4.6.1.2) (Atrial natriuretic peptide receptor type B) (ANP-B) (ANPR-B) (NPR-B) (Guanylate cyclase B) (GC-B), NPR2, ANPRB
Application:
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
19C7
Clonality:
Recombinant Monoclonal
Immunogen:
A synthesized peptide derived from Human NPR2
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing Hela cells stained with CSB-RA926985A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of Hela with CSB-RA926985A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 501-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot Positive WB detected in: HEPG2 whole cell lysate, MCF7 whole cell lysate All lanes: ANPRB antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 117 kDa Observed band size: 117 kDa